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HPLC Columns

Used Columns equipment for sale

HPLC Columns

HPLC Columns come in a variety of particle sizes and designs that meet different separation needs. The two important factors in choosing a column for your application are particle size and the internal diameter (ID) of the column.

Particle Size

The most common particle size is 5 µm silica beads. Smaller particles generally provide more surface area and better separations. However, decreasing the size of the particles while keeping the column ID size the same, while increasing the performance, will also increase the required pressure for optimum linear velocity. Larger particles are used in preparative HPLC and for non-HPLC applications such as solid-phase extraction.

Internal Diameter

The internal diameter (ID) of an HPLC Column influences the detection sensitivity and separation selectivity in gradient elution (steady changes of the mobile phase composition during the chromatographic run). Larger ID columns are usually seen in industrial applications, while small ID columns have better sensitivity and lower solvent consumption at the expense of loading capacity.

Top HPLC Columns Manufacturers

Typical manufacturers are Phenomenex, Agilent Technologies Inc., Supelco, and Waters Corporation. HiTechTrader has a multitude of HPLC Columns in various particle and ID sizes.

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We have been helping customers like you decide between different products since 1982 so send us an inquiry or call us today.

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5433 - Sepragen Corp. Superflo 5oo Column
Number of Items: 51

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010131101

Supelco Progel TSK Column Phenyl 5PW

    Price: $299.00    POD Price: $240.00    

Supelco Progel TSK Column Pheny-5PW, 7.5cm x 7.5mm. Brand new in sealed box.



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009131101

Supelco Progel TSK Column G300 SW

    Price: $349.00    POD Price: $280.00    

Supelco Progel TSK Column G300 SW 30cm x 7.5mm. Brand new in sealed box.



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006131015

Agilent Zorbax CombiHT HPLC Column XDB C8

    Price: $299.00    POD Price: $199.00    

Agilent Zorbax CombiHT HPLC Column XDB-C8. 5 um, 21.2mm id x 100 mm.



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005131015

Agilent Zorbax CombiHT HPLC Column 5B C8

    Price: $301.00    POD Price: $299.00    

Agilent Zorbax CombiHT HPLC Column 5B-C8. 5 um, 21.2mm id x 100 mm.



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004131015

Monochrom HPLC Column 0504 030G212

    Price: $298.00    POD Price: $249.00    

Monochrom HPLC Column 0504-030G212. 10u, C18



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003131015

Monochrom HPLC Column 0505-030G212

    Price: $348.00    POD Price: $299.00    

Monochrom HPLC Column 0505-030G212. 5u, C18



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002131015

Phenomenex 03R 4253 P0 HPLC Column

    Price: Please Call    

Phenomenex, 03R-4253-P0 HPLC Column, Luna 10u C18 (2) size: 60x 21.20 mm, Particle size 10u micron.



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001131015

Phenomenex 03R 4253 P0 HPLC Column

    Price: $385.00    POD Price: $299.00    

Phenomenex, 03R-4253-P0 HPLC Column, Luna 10u C18 (2) size: 60x 21.20 mm, Particle size 10u micron.



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008120709

Phenomenex 00G-4252-E0 HPLC Column

    Price: $300.00    POD Price: $200.00    

Phenomenex, 0GF-4252-E0 HPLC Column, Luna 5u C18 (2) size: 250 x 4.60 mm, Particle size 5 micron.



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006120709

Phenomenex 00A-4251-A0 HPLC Column

    Price: Please Call    

Phenomenex, 00A-4251-A0 HPLC Column, Luna 3u C18 (2) size: 30 x 1.00 mm, Particle size 3 micron.



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005120709

Phenomenex 00B-4043-E0 HPLC Column

    Price: Please Call    

Phenomenex, 00B-4043-E0 HPLC Column, Luna 5u C18(2), size: 50 x 2.00 mm, Particle size 5 micron.



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004120709

Phenomenex 00B-4043-E0 HPLC Column

    Price: Please Call    

Phenomenex, 00B-4043-E0 HPLC Column, Luna 5u C5 100A, size: 50 x 4.6 mm, Particle size 5 micron.



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003120709

Phenomenex 00A-4251-E0 HPLC Column

    Price: Please Call    

Phenomenex, 00A-4251-E0 HPLC Column, Luna 3u C18 (2) size: 30 x 4.60 mm, Particle size 3 micron.



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002120709

Phenomenex 00A-4252-E0 HPLC Column

    Price: Please Call    

Phenomenex, 00A-4252-E0 HPLC Column, Luna 5u C18 (2) size: 30 x 4.60 mm, Particle size 5 micron.



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003120706

Phenomenex 00B-4255-E0 HPLC Column

    Price: Please Call    

Phenomenex, 00B-4255-E0 HPLC Column, Luna 5u CN 100A, size: 50 x 4.6 mm, Particle size 5 micron.



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005120322

Waters RCM 25 x 10

    Price: $124.00    POD Price: $100.00    
Preparative Cartridge Module this device applies radial compression on PrePak 25 x 10 cartridges,

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025111021

Agilent Extend-C18 LC/MS Column 760450-902

    Price: $209.00    POD Price: $180.00    
Agilent Extend-C18 LC/MS Column, 5um, 2.1 x 50mm

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016111021

Agilent Zorbax Eclipse XDB-C18 HPLC Column

    Price: Please Call    
Agilent Zorbax Eclipse XDB-C18, 3.0 x 100 mm, 3.5 µm. The Agilent ZORBAX Eclipse XDB columns provide four bonded phase choices for method development optimization.

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015111021

Agilent Zorbax SB-CN HPLC Column

    Price: $229.00    POD Price: $195.00    
Agilent Zorbax SB-CN , 4.6 x 150mm.

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010111021

Waters YMC Hydroshpere C18 HPLC Column HS12S05 250WT

    Price: $224.00    POD Price: $190.00    
Waters YMC Hydroshpere C18 HPLC Column HS12S05-250WT. 5um 2.0 x 250mm. Hydrosphere C18 is based on highly inert, ultrapure, pH neutral silica making this the ideal phase for the analysis of basic pharmaceuticals and chelating compounds even under LC/MS conditions. The hydrophilic C18 surface enhances polar selectivity and affords the ability to separate polar compounds under highly aqueous mobile-phase conditions without loss of retention, efficiency or peak performance (hydrophobic collapse). . 5um, 4.6 x 150mm. ... More Info

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009111021

Waters Spherisorb CNRP 5um HPLC Column PSS830908

    Price: $209.00    POD Price: $175.00    
Waters Spherisorb CNRP 5um HPLC Analytical Column PSS830908. 5um, 4.6 x 150mm.

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008111021

Waters Atlantis Symmetry Shield RP 18 HPLC Column 186000700

    Price: $179.00    POD Price: $160.00    

Waters Symmetry Shield RP 18 HPLC Column 186000700 is 5um and 3.0 x 100mm. These reverse-phased columns are based on Waters patented embedded polar group technology that literally shields the silica's residual surface silanols from highly basic analytes.

... More Info

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006111021

Waters Atlantis HILIC Silica HPLC Column 186002030

    Price: $194.00    POD Price: $170.00    
Waters Atlantis HILIC Silica HPLC Column 186002030. 5um, 4.6 x 100mm. Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches. ... More Info

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005111021

Waters Atlantis T3 HPLC Column 186003747

    Price: $189.00    POD Price: $170.00    
Waters Atlantis T3HPLC Column 186003747. 5um, 4.6 x 150mm. With selective optimization of pore diameter, C18-ligand density and endcapping, Atlantis T3 columns provide paramount retention for polar compounds, while exhibiting industry leading peak shape and efficiency for all analytes as well as exceptional chemical stability across a wide pH range. ... More Info

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004111021

Waters Atlantis dC18 HPLC Column 186001303

    Price: $75.00    
Waters Atlantis dC18 HPLC Column 186001303. dC 18 3um, 3.0x 100mm. Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. The Atlantis dC18 columns feature di-functionally bonded C18 ligands that have been optimized for use with highly aqueous mobile phases, including 100% water. ... More Info

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003111021

Waters Atlantis HILIC Silica HPLC Column 186002029

    Price: $200.00    
Waters Atlantis HILIC Silica HPLC Column 186002029. 3um, 4.6 x 100mm. Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches. ... More Info

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018111020

Waters Atlantis dC18 HPLC Column 186001344

    Out of Stock    

Waters Atlantis dC18 HPLC Column 186001344. dC 18 5um, 4.6 x 150mm. Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. The Atlantis dC18 columns feature di-functionally bonded C18 ligands that have been optimized for use with highly aqueous mobile phases, including 100% water.

... More Info

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017111020

Waters Atlantis dC18 HPLC Column 186001340

    Price: Please Call    
Waters Atlantis dC18 HPLC Column 186001340. dC 18 5um, 4.6 x 100mm. Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. The Atlantis dC18 columns feature di-functionally bonded C18 ligands that have been optimized for use with highly aqueous mobile phases, including 100% water. ... More Info

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015111020

Phenomenex 00G-4375-B0-E0 HPLC Column

    Price: $149.00    POD Price: $120.00    

Phenomenex, 00G-4375-B0 HPLC Column, Synergi 4u Hydro-RP 80A size: 250 x 2.00 mm, Particle size 4 micron.



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014111020

Phenomenex 00A-4108-B0 HPLC Column

    Price: $224.00    POD Price: $190.00    
Phenomenex, 00A-4108-B0 HPLC Column, Columbus 5u C18 110A size: 30 x 2.00 mm,

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013111020

Phenomenex 00G-4336-B0-E0 HPLC Column

    Price: $219.00    POD Price: $185.00    
Phenomenex, 00G-4336-B0 HPLC Column, Synergi 4u POLAR-RP 80A size: 250 x 2.00 mm, Particle size 4 micron.

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012111020

Phenomenex 00G-3300-E0 HPLC Column

    Price: $209.00    POD Price: $185.00    

Phenomenex, 00G-3300-E0 HPLC Column, Prodigy 5 ODS (2) 125A size: 250 x 4.60 mm, Particle size 5 micron.



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011111020

Phenomenex 00F-4449-E0 HPLC Column

    Price: $219.00    POD Price: $190.00    
Phenomenex, 00F-4449-E0 HPLC Column, Luna 3u HILIC size: 150 x 4.60 mm, Particle size 3 micron.

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010111020

Phenomenex 00F-4254-E0 HPLC Column

    Price: $99.00    POD Price: $80.00    

Phenomenex, 00F-4254-E0 HPLC Column, Luna 3u CN 100A size: 150 x 4.60 mm, Particle size 3 micron.



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009111020

Phenomenex 00D-3125-E0 HPLC Column

    Price: $224.00    POD Price: $185.00    
Phenomenex, 00D-3125-E0 HPLC Column, Inertsil 5u ODS-2 150A size: 100 x 4.60 mm, Particle size 5 micron.

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008111020

Phenomenex 00B-4252-B0 HPLC Column

    Price: $209.00    POD Price: $170.00    
Phenomenex, 00B-4252-B0 HPLC Column, Luna 5u C18 (2) size: 100 x 2 mm, Particle size 5 micron.

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005111020

Phenomenex 00D-4187-E0 HPLC Column

    Price: $204.00    POD Price: $180.00    
Phenomenex, 00D-4187-E0 HPLC Column, Columbus 5u C8 110A size: 100 x 4.60 mm, Particle size 5 micron.

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004111020

Phenomenex 00B-4251-B0 HPLC Column

    Price: $189.00    POD Price: $170.00    
Phenomenex, 00B-4251-B0 HPLC Column, Luna 3u C18 (2) size: 50 x 2 mm, Particle size 3 micron.

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003111020

Phenomenex 00D-4251-B0 HPLC Column

    Price: $229.00    POD Price: $190.00    
Phenomenex, 00D-4251-B0 HPLC Column, Luna 3u C18 (2) size: 100 x 2 mm, Particle size 3 micron,

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002111020

Phenomenex 00D-4248-B0 HPLC Column

    Price: $214.00    POD Price: $185.00    
Phenomenex, 00D-4248-B0 HPLC Column, Luna 3u CB (2) size: 100 x 2 mm, Particle size 3 micron,

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001111020

Phenomenex 00D-4256-B0 HPLC Column

    Price: $199.00    POD Price: $175.00    
Phenomenex, 00D-4256-B0 HPLC Column, Luna 3u Phenyl-Hexyl size: 100 x 2 mm, Particle size 3 micron,

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006111018

Hamilton PRP 1 Polymeric HPLC Column

    Price: $229.00    POD Price: $190.00    
Hamilton PRP 1 Polymeric HPLC Column. PRP-1 5 µm 100 Å 2.1 x 150 mm.

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005111018

Hamilton PRP 1 Polymeric HPLC Column

    Price: $229.00    POD Price: $190.00    
Hamilton PRP 1 Polymeric HPLC Column. PRP-1 5 µm 100 Å 2.1 x 150 mm.

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001110916

Waters RCM 25x10, Inserts for Guard-Pak and PrepPak Columns

    Price: $119.00    POD Price: $96.00    
Extension tubes, 25 mm x 10 cm carridges. Inserts for Guard-Pak and PrepPak columns.

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005110506

Upchurch Scientific 1322 Uptight Tubing Kit

    Price: $59.00    POD Price: $50.00    
Upchurch Scientific Uptight Tubing Kit Model 1322; 0.007" inner diameter x 10cm length U-127, 0.007" inner diameter x 5cm length U-126

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004110506

Upchurch Scientific 1323 Uptight Tubing Kit

    Price: $64.00    POD Price: $55.00    
Upchurch Scientific Uptight Tubing Kit Model 1323; 0.005" inner diameter x 10cm length U-153, 0.005" inner diameter x 5cm length U-152

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003110506

Upchurch Scientific 1323 Uptight Tubing Kit

    Price: $59.00    POD Price: $50.00    
Upchurch Scientific Uptight Tubing Kit Model 1323; 0.005" inner diameter x 10cm length U-153, 0.005" inner diameter x 5cm length U-152

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007090611

Waters Prepack Column Module Model 40C

    Price: Please Call    
Waters Prepack Column Module Model 40C 40 x 10 Chamber.

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019080730

Waters HPLC Column Mode PrepPak with RCM Base

    Price: Please Call    
Waters HPLC Column Mode PrepPak with RCM Base

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017050121

Waters RCM 25 x 10

    Price: $124.00    POD Price: $100.00    

Preparative cartridge Module this device applies radial compression on PrePak 25 x 10 cartridges,



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018050121

Waters RCM 25x10

    Price: $119.00    POD Price: $96.00    
Extension tubes, 25 mm x 10 cm carridges. Inserts for Guard-Pak and PrepPak columns.

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